美国加州大学圣迭戈分校任兵团队绘制出人脑中单细胞染色质可及性的较量图谱。该项研究效果揭晓在2023年10月13日出书的《科学》上。
使用通过测序的转座酶可及染色质（snATAC-seq）的单核剖析，研究职员探索了来自3名成年人的42个大脑区域的110万个细胞的开放染色质景观。整合这些数据展现了107种差别的细胞类型及其对人类基因组中544735个候选顺式调控DNA元件（cCRE）的特异性使用。近三分之一的cCRE在单核细胞中体现出守旧性和染色质可及性。研究职员展现了特定脑细胞类型与神经精神疾�。òň衿扑橹ⅰ⑺嗲樾髡习⒍暮Ｄ。ˋD）和重度抑郁症）之间的亲近联系，并开发了深度学习模子来展望非编码危害变异在这些疾病中的调理作用。
据相识，单细胞转录组学的最新希望展现了人类大脑中差别的神经元和胶质细胞类型。然而，调控细胞身份和功效的调控程序仍不清晰。
附：英文原文
Title: A comparative atlas of single-cell chromatin accessibility in the human brain
Author: Yang Eric Li, Sebastian Preissl, Michael Miller, Nicholas D. Johnson, Zihan Wang, Henry Jiao, Chenxu Zhu, Zhaoning Wang, Yang Xie, Olivier Poirion, Colin Kern, Antonio Pinto-Duarte, Wei Tian, Kimberly Siletti, Nora Emerson, Julia Osteen, Jacinta Lucero, Lin Lin, Qian Yang, Quan Zhu, Nathan Zemke, Sarah Espinoza, Anna Marie Yanny, Julie Nyhus, Nick Dee, Tamara Casper, Nadiya Shapovalova, Daniel Hirschstein, Rebecca D. Hodge, Sten Linnarsson, Trygve Bakken, Boaz Levi, C. Dirk Keene, Jingbo Shang, Ed Lein, Allen Wang, M. Margarita Behrens, Joseph R. Ecker, Bing Ren
Issue&Volume: 2023-10-13
Abstract: Recent advances in single-cell transcriptomics have illuminated the diverse neuronal and glial cell types within the human brain. However, the regulatory programs governing cell identity and function remain unclear. Using a single-nucleus assay for transposase-accessible chromatin using sequencing (snATAC-seq), we explored open chromatin landscapes across 1.1 million cells in 42 brain regions from three adults. Integrating this data unveiled 107 distinct cell types and their specific utilization of 544,735 candidate cis-regulatory DNA elements (cCREs) in the human genome. Nearly a third of the cCREs demonstrated conservation and chromatin accessibility in the mouse brain cells. We reveal strong links between specific brain cell types and neuropsychiatric disorders including schizophrenia, bipolar disorder, Alzheimer’s disease (AD), and major depression, and have developed deep learning models to predict the regulatory roles of noncoding risk variants in these disorders.
DOI: adf7044
Source: https://www.science.org/doi/10.1126/science.adf7044
期刊信息
Science：《科学》，创刊于1880年。隶属于美国科学增进会，最新IF：63.714
官方网址：https://www.sciencemag.org/
投稿链接：https://cts.sciencemag.org/scc/
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